Gene	Primer sequence (5′-3′)	AT (°C)	PS (bp)
FAM46B	F-CCTGCCTACTAGACTTCCTGC	60	149
	R-CTTGCCGCTCTTGTTGGACA
OCT4	F-AGTGAGAGGCAACCTGGAGA	60	81
	R-ACACTCGGACCACATCCTTC
NANOG	F-CATGAGTGTGGATCCAGCTTG	60	191
	R-CCTGAATAAGCAGATCCATGG
SOX2	F-GGGAAATGGGAGGGGTGCAAAAGAGG	60	151
	R-TTGCGTGAGTGTGGATGGGATTGTG
PBGD	F-ATTACCCCGGGAGACTGAAC	60	130
	R-GGCTGTTGCTTGGACTTCTC
SOX1	F-ACCAGGCCATGGATGAAG	60	67
	R-CTTAATTGCTGGGGAATTGG
GATA4	F-GGAAGCCCAAGAACCTGAAT	60	98
	R-GTTGCTGGAGTTGCTGGAA
T	F-ACCCAGTTCATAGCGGTGAC	60	165
	R-CCATTGGGAGTACCCAGGTT
GAPDH	F-AGCCACATCGCTCAGACAC	60	66
	R-GCCCAATACGACCAAATCC

F, forward; R, reverse; AT, annealing temperature; PS, product size.