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| Gene | Primer sequence (5′-3′) | AT (°C) | PS (bp) |
|---|---|---|---|
| Wnt5A | F-CCCTTAGGCAGGTTGGCTTT | 60 | 90 |
| R-CACTGCAACTGAGGGGATGT | |||
| RICTOR | F-TGCAGAAGCACCCTTCACAA | 60 | 148 |
| R-AGCCAGTTATAGCACAACCCC | |||
| SKP2 | F-TGACATCGGATGCCCTCAAA | 60 | 249 |
| R-AAGCCAAATGGCATACCACAC | |||
| PAPBC4 | F-TGCAAGGTGGTGTGTGATGA | 60 | 70 |
| R- CAGCCTCTTGGGTCTCGAAG | |||
| RPS25 | F-GAAGATTCGAGGCTCCCTGG | 60 | 128 |
| R-CTGGAGCATCTCCACCCTTG |
F, forward; R, reverse; AT, annealing temperature; PS, product size.
