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. 2020 Jan 20;48(5):e30. doi: 10.1093/nar/gkaa017

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Examination of Cas12a for its capability of nonspecific trans-ssDNA cleavage and compatibility with PG-RCA. (A) Agarose gel electrophoresis analysis for the PG-RCA and CIRI. The red arrow indicates the direction of electrophoresis. (B) Real-time changes of (1–10 Hz) for disassembly of MNP clusters by Cas12a ternary complexes. Working concentrations of Cas12a are indicated in the plot. (C) Real-time changes of (1–10 Hz) for disassembly of MNP clusters by PG-RCA activated Cas12a. Target B molecules of different concentrations (indicated in the plot) were added as the primer of RL. Repeated measurements for the same reaction are plotted in the same color.

Inline graphic — Examination of Cas12a for its capability of nonspecific trans-ssDNA cleavage and compatibility with PG-RCA. (A) Agarose gel electrophoresis analysis for the PG-RCA and CIRI. The red arrow indicates the direction of electrophoresis. (B) Real-time changes of (1–10 Hz) for disassembly of MNP clusters by Cas12a ternary complexes. Working concentrations of Cas12a are indicated in the plot. (C) Real-time changes of (1–10 Hz) for disassembly of MNP clusters by PG-RCA activated Cas12a. Target B molecules of different concentrations (indicated in the plot) were added as the primer of RL. Repeated measurements for the same reaction are plotted in the same color.