Figure 2.

Detection of NA_RNA depends on the sequencing method. (A) Viral based sequencing library protocols poorly detect most of the NA_RNA. The abundance of 111 NA_RNA in three different RNA-Seq library preparation methods was compared and the unsupervised clusters shown in the form of a heatmap. All sequencing methods were generated using ribo-depleted SKOV3ip1 RNA. Color legend for abundance is given at the top (ND: not detected). The sequencing methods are indicated at the bottom. Gene biotype colors are indicated on the right of panel B. (B) Violin plots of the distribution of NA_RNA abundance as detected in non-fragmented SKOV3ip1 datasets are shown for each biotype. The color legend for biotypes is on the right.