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. 2020 Jan 25;48(5):2271–2286. doi: 10.1093/nar/gkaa028

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — The newly identified tRNA fragments are generated from canonical tRNA genes. (A) Alignment of the sequence of cluster_471 with the sequence of its most similar tRNA gene (tRNA-lys URS0000206F1C). Identical nucleotides are indicated by asterisks and mismatches by blank spaces; the fragment sequence is highlighted in green. (B) Predicted structure of the RNA fragment cluster_471 in the context of the mature RNA of its reference gene. The fragment sequence is highlighted in green. (C) Distribution of the size and position of the NA_tRNA fragments relative to their reference genes. 0 and 1 indicate the mature 5′ and 3′ ends of the reference gene, respectively. The ratio of fragments over reference gene length is indicated on the right. The asterisk indicates incomplete reference tRNA that may not fold into mature tRNA structure.