Figure 2.

Lysine-acetylation of HrdB during growth. The HrdB proteins were immunoprecipitated with anti-HrdB antibody from Sven15439 cell extracts (2 mg) prepared at early- (E), mid- (M) and late- (L) exponential and stationary (S) phases. Precipitated proteins (HrdB-IP) (3 μl for HrdB and 15 μl for AcK) were analyzed by western blot using antibodies against HrdB or acetylated-lysine (AcK). For each immunoblot, the levels were measured by densitometric analyses of the western blots and presented as the relative values to the level at E phase. The data represent average values with standard errors from four independent experiments.