Skip to main content
. 2020 Jan 23;48(5):2401–2411. doi: 10.1093/nar/gkaa011

Figure 6.

Figure 6.

Effect of K259 mutations on HrdB binding to RNAP core enzyme. (A) Expression levels of FLAG-tagged HrdB proteins with a K259 mutation to either glutamine (JE02-Q) or arginine (JE02-R) in comparison with that of the wild type (JE02). Cell extracts (5 μg) at the early exponential phase were analyzed by western blot using anti-FLAG antibody. (B) Effect of mutations on the association of HrdB with the RNAP core enzyme. The same cell samples of A were lysed and immunoprecipitated with anti-FLAG to collect HrdB and its associated proteins. Precipitates (HrdB-IP) (4, 2 and 0.5 μg for AcK, HrdB and RpoB, respectively) were analysed by western blots using anti-AcK, anti-FLAG, or anti-RpoB antibodies. Representative images from three independent experiments are shown. (C) The quantification of the data in (B). The RpoB/HrdB ratios relative to that in JE02 are shown with error bars representing the standard errors from three independent experiments. Statistical significance between the groups is indicated, based on a P value of <0.05 (*) by using Student's t-test. The difference between JE02 and JE02-Q is not statistically significant (n.s.).