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. 2020 Jan 25;48(5):2486–2501. doi: 10.1093/nar/gkz1218

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Cascade/I-C and Cas3/I-C form a stable complex during interference. (A) Schematic representation of 100 bp DNA substrates with biotin (black dot) at 5′ end of the non-target strand (NTS), 6-FAM (shown as green star) at 5th nucleotide, Iowa Black® FQ (shown as brown dot) at 28th nucleotide on target strand (TS) and PAM sequence TTC (depicted in red). (B) Substrate mentioned above was incubated with or without Cascade/I-C (1 μM) and increasing concentration of Cas3/I-C (0–5 μM). A significant decline in fluorescence intensity is evident when both Cascade/I-C and Cas3/I-C were present. There was no apparent quenching when dsDNA and ssDNA were used in the absence of Cascade/I-C. (C) Fluorescence quenching was observed in the presence of ATP but not when ADP and AMP-PNP were used.