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. 2020 Jan 20;48(5):2579–2593. doi: 10.1093/nar/gkaa018

Figure 2.

Figure 2.

The 5p-strand single cleavage products of pri-miRNAs are intrinsically resulted from Microprocessor catalysis. (A) The purified wild-type (WT) and mutant D3-G1 complexes analyzed by SDS-PAGE. (B and C) Pri-miRNA processing assays. Five pmol of pri-mir-16-1 (B) or pri-mir-92a-1 (C) were incubated with 8 pmol each of D3-G1 and the G4 dimer for 2 h at 37°C. (D) The purified D3-DG4 complex was fractionated by a gel filtration column (Superdex 200 Increase 10/300 GL) in the NGC system (Bio-Rad) and was analyzed by SDS-PAGE. (E and F) Pri-miRNA processing assays. Five pmol of pri-mir-16-1 (E) or pri-mir-92a-1 (F) were incubated with 3 μl of each D3-DG4 fraction from (D) for 2 h at 37°C.