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. 2020 Jan 20;48(5):2579–2593. doi: 10.1093/nar/gkaa018

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — The size of asymmetric internal loop in the lower stem affects the level of the 5p-strand single cleavage of Microprocessor. (A and D) Diagram of pri-mir-92a-1 and its variants. The mutated nucleotides are in red. (B and E) Pri-miRNA processing assays. Five pmol of each pri-miRNA were incubated with 8 pmol each of D3-G1 and the G4 dimer for 2 h at 37°C. (C) Quantification of the pri-miRNA processing data from (B). The pri-miRNA processing assays in (B) were repeated three times. The single-cut (sc) to double-cut (dc) activity ratio (sc/dc ratio) was estimated as the ratio of the single-cut to double-cut product (F2) band density in the gel. The p-values of the one-tailed t-test for the sc/dc ratios estimated from three replicates were shown. MP: Microprocessor.