Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jan 24;48(5):2594–2603. doi: 10.1093/nar/gkaa023

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 6. — Heterologous cooperation assay. (A) DNA substrate used for the assay. The blue arrows illustrates the location of DNA cleavage by LlaGI-LlaBIII and LlaGI-LlaBIII^R564A based on the data in B and C. (B, C) DNA cleavage assay showing LlaGI-LlaBIII shreds the dsDNA, leading to a smear ranging from 1400 to 2200 bp whereas LlaGI-LlaBIII^R564A cleavage products are close to 1400 and 1000 bp. 10 nM DNA was incubated with 250 nM enzyme. The reaction was started by addition of 4 mM ATP.