Table 1.

Kinetic and thermodynamic parameters for Zur–DNA interaction in E. coli cells (error bars are s.d.)

	ZurmE
k 1(nM−1 s−1)a	0.80 ± 0.07	0.77 ± 0.08	0.46 ± 0.08	0.68 ± 0.24	0.55 ± 0.08
(s−1)	25 ± 12	22 ± 21	5.4 ± 0.6	22 ± 2	36 ± 41
k r (s−1)	16 ± 11	12 ± 20	n/ob	21 ± 1	27 ± 40
k f (nM−1 s−1)	0.012 ± 0.002	0.018 ± 0.003	0.011 ± 0.014	0.021 ± 0.006	0.026 ± 0.004
K m (nM)	14 ± 10	12 ± 17	n/ob	39 ± 18	7.6 ± 4.5
K d1 ( =/k1) (nM)a	31 ± 15	28 ± 27	12 ± 3	33 ± 12	67 ± 48
K d2 ( = k-2/k2) (nM)a	990 ± 80	830 ± 200	1300 ± 400	500 ± 160	1300 ± 300
K d3 ( = k-3/k3)a	0.011 ± 0.002	0.023 ± 0.007	0.022 ± 0.023	0.032 ± 0.062	0.008 ± 0.006
[D0]NB (nM)a	2700 ± 200	2300 ± 500	2900 ± 700	2000 ± 500	3700 ± 800
[D0]TB⋅no (nM)a	100 ± 2	82 ± 8	130 ± 30	75 ± 12	92 ± 9

^a n _o = 5 was used in fitting; see Supplementary Figure S15 for n_o dependence of parameters.

[D₀]_NB: concentration of nonspecific binding sites in cell. [D₀]_TB: concentration of tight binding sites in cell.

^bNot observed.