Figure 2.

Enrichment of insertions for H-NS and other global regulators. (A) Z-score of enrichment tests for different gene lists (see Supplementary Table S1 for a full legend). H-NS binding sites (from ChIP-seq and ChIp-ChIP data) and H-NS perturbations experiments (from (39)) are highly enriched (circles), indicating a strong positive association of insertions to H-NS binding regions starting from the parental colony. Other global nucleoid regulators (FNR, Fis, IHF, CRP, see legend), and a list of horizontal transfer genes (HT, see legend) also show positive association, lists of essential genes (filled squares) show strong negative enrichment. (B) Comparison of the two different promoter tested (with and without Fis and H-NS binding sites) shows a similar behaviour. (C) Comparison of parental and sorted populations (see Figure 1C–E) shows that H-NS association maintains a strong significance in the low-expression population, and loses significance in the high-expression population, where FNR sites remain highly enriched.