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. 2020 Jan 16;48(5):2709–2722. doi: 10.1093/nar/gkz1224

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© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 9. — Effect of aptamers on protease cleavage of p66. Aptamer used in this assay were the RNA aptamer 148.1-38m and DNA aptamer RT1t49(-5). (A) Representative protein immunoblot showing PR-mediated cleavage of p66/p66. The presence of p66 and p51 in each sample was detected by Western blot. Free p66/p66 RT was used as a control for background signal of p51. Additional data can be found in Supplementary Figure 9. (B) Quantitative representation of three individual experiments. Intensities of p66 and p51 bands corresponding to each reaction sample were quantified using ImageJ then the p51:p66 ratio was calculated. The p51:p66 ratio correlated with free p66/p66 was set as background and was subtracted from the p51:p66 ratio of each remaining sample. The adjusted p51:p66 ratio then was plotted via Prism software version 6.2. ns (P > 0.05), * (P < 0.05). Values shown represent triplicate measurements (±SD) of three independent experiments.