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. 2020 Feb 26;14:833–844. doi: 10.2147/DDDT.S224343

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© 2020 Chen et al.

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FGFR1 activation is upregulated in kidney tissues in LPS mice. (A) Western blot and densitometric analysis of P-FGFR1 and FGFR1 levels in kidney tissues [n=5 per group; 2 Ctrl, 2 LPS and 2 LPS+AZD samples shown in immunoblots]. (B) Immunofluorescence staining for P-FGFR1 (red) and AQO-1 (green) showing immunoreactivity of P-FGFR1 in tubular epithelial cells [scale bar = 50 μm]. Merged images (yellow) showing colocalization. (C) Immunofluorescence staining of kidney tissues for P-FGFR1 (red) and WT-1 (green) [scale bar = 50 μm]. WT-1 was used as a marker of podocytes. (*, vs Ctrl group; #, vs LPS group; ^#P<0.05, **P<0.01.)