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. 2020 Feb 26;14:833–844. doi: 10.2147/DDDT.S224343

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© 2020 Chen et al.

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AZD4547 modulates TAK1/NF-κB signaling by increasing the interaction between P-FGFR1 and TRAF6. (A) Ubiquitination was carried out in the presence of TRAF6 and blocked by AZD4547 in a dose-dependent manner. Immunoblotting was performed with an anti-Ub antibody. (B) Lysates from LPS-stimulated (indicated times) NRK-52E cells were immunoprecipitated with TRAF6 antibody (IP) and immunoblotted (IB) for P-FGFR1. (C) Immunoprecipitation (IP) of lysates from LPS-stimulated (30 min) NRK-52E cells with AZD4547; IP of TRAF6 and immunoblotting (IB) for P-FGFR1 proteins [n= 3 per group]. (D) Schematic illustration of the potential mechanisms: LPS activates FGFR1 and increases the interaction between P-FGFR1 and TRAF6 to produce TAK1/NF-κB signaling activation and the production of inflammatory cytokines. These inflammatory deficits cause acute kidney injury. (*, vs Ctrl group; #, vs LPS group; *** and ^###P<0.001.)