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. 2020 Feb 21;11:238. doi: 10.3389/fimmu.2020.00238

Figure 3.

Figure 3

Reduced STING protein expression in STING-HAQ but not STING-R231H B cells. (A,B) STING protein expression in WT, STING+/−, STING−/−, STING-HAQ/WT, STING-HAQ/HAQ, STING-R231H/WT, and STING-R231H/R231H B cells. A representative blot is shown in (A), normalized STING expression (n = 6/genotype) is shown in (B). (C) WT, STING+/−, STING KO, STING-HAQ/WT, and STING-R231H/WT B cells (n = 4) were cultured for 18 h with the indicated concentrations of CDG. CD86 expression (MFI gated on B220+ Annexin V−) is plotted (top graph) and B cell survival (% B220+ Annexin V− cells) after stimulation is plotted in the bottom graph. (D) WT, STING KO, STING-HAQ/HAQ, and STING-R231H/R231H B cells (n = 4) were cultured for 18 h with IL4 and CD86 expression (MFI) was measured. Data shown are representative of at least two independent replicate experiments. Bars in (A–D) represent mean ± SEM. Statistical comparisons were made for each stimulus concentration and the range of concentrations that reached statistical significance between genotypes are indicated by horizontal lines above the graph. *P < 0.05; **P < 0.01 using an unpaired Student's t-test.