Figure 6.

STING-R231H homozygous mice are unresponsive to immunization using CDG as an adjuvant but remain responsive to immunization using 2′3′ cGAMP as an adjuvant. (A–G) WT, STING KO and STING-R231H/WT and STING-R231H/R231H were immunized IN with 20 μg OVA with or without 5 μg CDG (B,D,F) or 5 μg 2′3′ cGAMP (C,E,G), and serum and mucosal anti-OVA antibody responses were monitored (n = 5). (A) Schematic representation of the experiment; (B,C) IgG anti-OVA response at d42 post-immunization, (D,E) Reciprocal half-max values of the OVA-specific IgG response; (F,G) OVA-specific IgA response in nasal wash at day 56. Data shown are representative of at least two independent replicate experiments. Bars in B-G represent mean ± SEM. NS, P > 0.05; ***P < 0.001 using an unpaired Student's t-test.