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. 2020 Jan 27;39(5):e102622. doi: 10.15252/embj.2019102622

Figure EV1. Mapping the CaV1.2 IQ binding site in α‐actinin and apoCaM.

Figure EV1

  1. Overlay of 15N‐1H HSQC spectra of 15N‐labeled α‐actinin‐1 CH1/CH2 domain (residues 19–192) by itself (black peaks) and after addition of saturating, unlabeled IQ peptide (red peaks).
  2. Overlay of 15N‐1H HSQC spectra of 15N‐labeled α‐actinin‐1 EF‐hand domain (residues 750–892) by itself (black peaks) and after addition of saturating, unlabeled IQ peptide (red peaks).
  3. Overlay of 15N‐1H HSQC spectra of 15N‐labeled α‐actinin‐1 C‐lobe (α‐actinin‐1 EF34; residues 822–892) by itself (black peaks) and after addition of saturating, unlabeled IQ peptide (red peaks).
  4. Overlay of 15N‐1H HSQC spectra of 15N‐labeled apoCaM by itself (black peaks) and after addition of saturating, unlabeled IQ peptide (red peaks).