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. 2020 Jan 27;39(5):e102622. doi: 10.15252/embj.2019102622

Figure 4. Ectopic α‐actinin‐1 expression increases CaV1.2 open probability.

Figure 4

HEK293 cells were transfected with α11.2, α2δ‐1, and β2A plus, if indicated, α‐actinin‐1 before cell‐attached patch recording.
  • A
    Representative single CaV1.2 channel recordings of WT CaV1.2 alone or with WT or E847K/E851K mutant α‐actinin‐1.
  • B
    Mean assemble averages for all experiments for each combination.
  • C–G
    Dot plots and means ± SEM for availability (C), NPo (D), Po (E), unitary current amplitude i (F), and the mean of the current of a single channel calculated from the ensemble averages of each experiment (G) (*P < 0.05, **P < 0.01, ****P < 0.001; unpaired t‐test; n = 13–35; see Table 6 for more details).