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. 2020 Feb 3;39(5):e103444. doi: 10.15252/embj.2019103444

Figure EV1. The “downstream osmosensor” responds to osmostress regardless of the type of osmostressor.

Figure EV1

  1. Conversion of NaCl and sorbitol solute concentration (molarity, M) to osmotic pressure Π (mega pascal, MPa). For more detail, see the Appendix Fig S1.
  2. Comparison of Hog1 phosphorylation induced by NaCl and sorbitol. The yeast strain TM142 (WT) was stimulated with various concentrations of NaCl or sorbitol for 5 min, and the percentage of Hog1‐P was determined using a Phos‐tag band‐shift assay. Solution molar concentrations were converted to the corresponding osmotic pressures (MPa) using the table in (A).
  3. Phos‐tag band‐shift analyses of sorbitol‐induced Hog1 phosphorylation. The yeast strain KY603‐3 (ΔS/O/H/M ssk2/22Δ STE11‐Q301P) was stimulated with the indicated concentrations of sorbitol for 5 min.
  4. Same as in (B), except that the yeast strain KY603‐3 (ΔS/O/H/M ssk2/22Δ STE11‐Q301P) was used.

Source data are available online for this figure.