Figure 4. Inhibition of the osmotic enhancement of the Pbs2‐Hog1 reaction affects the SLN1 and SHO1 branches differently.

• A
  Phos‐tag band‐shift analyses of osmostress‐induced Hog1 phosphorylation. The yeast strain FP4 (hog1Δ) was transformed with the single‐copy expression plasmid pRS416‐Hog1‐ΔL16 and was stimulated with the indicated concentrations of NaCl for 5 min.
• B
  Averages of three independent experiments from A were plotted. Results for Hog1‐WT (from Fig 1I) are included for comparison.
• C, D
  Same as in (A and B), except that the yeast strain KT259 (ste11Δ hog1Δ) was used.
• E, F
  Same as in (A and B), except that the yeast strain KY523 (ssk2/22Δ hog1Δ) was used.

Data information: (A, C, and E) Representative results from three independent experiments. (B, D, and F) Error bars are SEM (n = 3).Source data are available online for this figure.