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. 2020 Feb 18;2020:9018624. doi: 10.1155/2020/9018624

Figure 2.

Figure 2

Effect of sevoflurane exposure on the expression of SIRT1 and BDNF in vivo and in vitro. (a, b) RT-PCR analysis of SIRT1 and BDNF mRNA expression between the control group and the sevoflurane group in hippocampal tissue, respectively (n = 3 per group). (c) Representative western blot bands of SIRT1 and BDNF in the neonatal mouse hippocampus (n = 4 per group). (d, e) Densitometry quantification of SIRT1 and BDNF expression ex vivo, respectively. (f) Representative western blot bands of SIRT1 and BDNF in primary hippocampal neurons (n = 4 per group). (g, h) Densitometry quantification of SIRT1 and BDNF expression in vitro, respectively. Data are shown as means ± SEM (n = 4 per group). ∗P < 0.05, ∗∗P < 0.01.