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. 2020 Feb 18;9(1):1727637. doi: 10.1080/20013078.2020.1727637

Figure 4.

Figure 4.

Systems biology analysis of proteins identified by Shot-gun proteomic derived from microglia sEVs. (a) Venn diagram of proteins of sEVs released by microglia. (b) Proteins identified in proteomics data (Cx-M sEVs and SpC-M sEVs) also listed in top 50 proteins that are often identified in EVs. Pathway analyses of (c) common protein from sEVs of Cx-M treated or not with 500 ng/mL LPS for 24 h (d) specific proteins from sEVs of Cx-M cells treated with 500 ng/mL LPS for 24 h (e) common protein from sEVs of SpC-M treated or not with 500 ng/mL LPS for 24 h and (f) common protein of sEVs released cortex and SpC-M treated with LPS. (g) Heatmap from shot proteomic analysis using MaxQuant after ANOVA with a p value > 0.05 for sEVs released by microglia isolated from two different sources, cortex and spinal cord, and pathways issued from systemic biology analyses of cluster 1a,1b, 2 and 3 from the heatmap. The analyses were performed in replicate (n = 3).