FIG. 4.

Dystrophin levels (% of WT) in quadriceps and gastrocnemius of SC and IV dosed mdx mice, assessed using Wes (A) and IFA (B). For Wes, rabbit monoclonal anti-dystrophin antibody (no. ab154168; Abcam), and for IFA, rabbit polyclonal anti-dystrophin antibody (no. ab15277; Abcam) were used. Values are presented as mean ± SEM. Dots represent values obtained in individual mice (n = 14–15). Significance between the mdx groups was assessed using ANOVA and Tukey's multiple comparison test (*P < 0.05, **P < 0.01, ****P < 0.0001). (C) Representative IFA images are shown of quadriceps and gastrocnemius for M23D-treated mdx mice in the SC and IV groups in comparison to the scrambled control group. IFA, immunofluorescence analysis; Wes, capillary Western immunoassay.