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. 2020 Jan 29;295(9):2724–2735. doi: 10.1074/jbc.RA119.010719

Figure 1.

Figure 1.

DSCR6 binds to the C-terminal region of Stat3. A, schema shows the full-length and truncated Stat3 proteins used for analysis of interaction with DCSR6. Myc, 6 myc epitopes at the N terminus; Tetra, domain involved in Stat3 tetramerization; Coiled-coil, protein interaction domain; DBD, DNA-binding domain; Linker, linker region; SH2, Src homology 2; TAD, transactivation domain. B, temporal interaction between endogenous DSCR6 and Stat3 in blastula (stage 7), early gastrula (stage 10), and late gastrula (stage 12), analyzed by co-immunoprecipitation using anti-DSCR6 antibody. C, GST pulldown of in vitro translated Stat3 by GST-DSCR6. D, GST pulldown of myc-tagged full-length and truncated Stat3 proteins expressed in the embryos by GST-DSCR6. E–H, immunofluorescence staining using anti-DSCR6 antibody shows the expression of endogenous DSCR6 protein in the cytoplasm and nuclei of all three germ layers in blastula (stages 8 and 9), early gastrula (stage 10), and mid-gastrula (stage 11). The sections are oriented with animal pole region up and dorsal region on the right. Scale bar: E–H, 200 μm.