Figure 4.

Ezh2 binds to the C-terminal region of Stat3. A, Western blot analysis shows the temporal expression of Ezh2 from cleavage to tail-bud stages. Tubulin is a loading control. B–E, apotome microscopic analysis shows the nuclear localization of Ezh2 in late blastula (stage 9) and mid-gastrula (stage 11). F, temporal interaction between endogenous Ezh2 and Stat3 in blastula (stage 7), early gastrula (stage 10), and late gastrula (stage 12), analyzed by co-immunoprecipitation using anti-Ezh2 antibody. G, GST pulldown of in vitro translated myc-tagged Stat3 by GST-Ezh2. H, GST pulldown of full-length and truncated Stat3 proteins expressed in the embryos by GST-Ezh2. Scale bar: B–E, 200 μm.