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. 2020 Jan 29;295(9):2724–2735. doi: 10.1074/jbc.RA119.010719

Figure 5.

Figure 5.

Functional interaction between Ezh2 and Stat3 in DV patterning. A–D″, in situ hybridization analysis shows the expression of dorsal (chordin and otx2) and ventral (wnt8 and Xvent1) mesoderm markers in early gastrula expressing Ezh2-H694L or Ezh2-H694L and Stat3C in the ventral region. Arrows indicate ectopic expression of chordin and otx2; arrowheads show the region where wnt8 and Xvent1 expression is repressed. All embryos are vegetal view with dorsal region up. E–G, live images of tail-bud stage embryos. Activation of Stat3 inhibits the formation of secondary axis (II) induced by ventral expression of Ezh2-H694L. H, Western blot (WB) analysis following immunoprecipitation shows the inhibition of Stat3 methylation by Ezh2-H694L at early gastrula stage. I, quantification of lysine-methylated Stat3 level (***, p < 0.001). J, luciferase reporter assay for Stat3 transcriptional activity in early gastrula-stage embryos expressing Ezh2 or Ezh2-H694L (NS, not significant; ***, p < 0.001). Scale bars: A–D″, 500 μm; E–G, 500 μm.