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. 2020 Jan 29;295(9):2724–2735. doi: 10.1074/jbc.RA119.010719

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© 2020 Loreti et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 6. — Ezh2 phosphorylation is required for Stat3 methylation and transcriptional activity. A and B, analysis of the nuclear localization of Ezh2-S21P in the large-cell blastula (stage 7) and early gastrula (stage 10). C, luciferase reporter assay of Stat3 transcriptional activity in early gastrula expressing a constitutively active form of Ezh2, Ezh2-S21D, or a nonphosphorylable form of Ezh2, Ezh2-S21A (*, p < 0.05; **, p < 0.01). D, a control embryo at tail-bud stage. E, ventral expression of Ezh2-S21D does not affect embryonic development. F and G, ventral expression of Ezh2-S21A induces a secondary axis with somitic tissue. H and I, ventral expression of an inhibitory form of Stat3 methylation, Stat3-K49R, induces a secondary axis with somitic tissue. Scale bars: A and B, 200 μm; D–F and H, 500 μm; G and I, 500 μm.