Figure 1.

Oligomerization states of full-length adiponectin. A, size exclusion chromatography of full-length adiponectin shows three peaks at three different retention volumes. B, the three peaks from the size exclusion chromatography were run on nonreducing SDS-PAGE and stained with Coomassie dye. C, two-dimensional negative-stain EM class averages show the adiponectin oligomerization state of each peak from size exclusion chromatography. Each image has the same scale, but different circular masks were used for classification according to the size of the particle. Scale bar = 20 nm.