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Each mutation independently enhances T-cadherin binding to the adiponectin globular domain on yeast. Each adiponectin (AQ) mutant was tested for its binding to purified T-cadherin (100 nm) in a yeast surface display assay. On the x axis, T-cadherin binding is shown, whereas on the y axis, adiponectin expression is reported. The upper right quadrant indicates yeasts that express adiponectin and bind T-cadherin.
