Skip to main content
. 2020 Jan 28;295(9):2713–2723. doi: 10.1074/jbc.RA119.010683

Figure 1.

Figure 1.

C. pneumoniae infection induces the secretion of FABP4 from murine adipocytes. A, immunoblot analysis of FABP4 in cultured medium (CM) and cell lysates (CL) of 3T3-L1 adipocytes after mock or C. pneumoniae (Cpn) infection for 2–24 h. β-Actin served as the standard. B, secretion of FABP4 was measured in the cultured medium of 3T3-L1 adipocytes after mock or Cpn infection for 2–24 h. A 4-h incubation with forskolin (20 μm) served as the positive control for lipolysis. C, relative levels of Fabp4 mRNA in 3T3-L1 adipocytes after mock or Cpn infection for 4–24 h, as determined by real-time PCR. Gus mRNA served as the internal control. D, LDH assay using the supernatant of 3T3-L1 adipocytes at 2–24 h after mock or Cpn infection (n = 3/group; B–D). **, p < 0.01 by two-way ANOVA (B and C). The data are shown as the means ± S.E. and are representative of at least three experiments.