Figure 7.

C. pneumoniae usurps infection-induced ER stress/UPR and subsequent elevation of mitochondrial ROS for its replication. A–D, the number of infectious EB progeny of 3T3-L1 adipocytes at 24 h after Cpn infection at a MOI of 5 in the presence or absence of MitoTEMPO (A, 100 μm), MitoQ (B, 1 or 5 μm), azoramide (C, 20 μm to 50 μm), GSK2606414 (D, PERK inhibitor, 2 μm), or STF-083010 (IRE1α RNase-specific inhibitor, 50 μm) was determined using an IFU assay. E, at 48 h after transfection (siCHOP, siPERK, or siControl), 3T3-L1 adipocytes were infected with Cpn MOI 5 for 24 h, and infectious EB progeny was determined using an IFU assay (n = 3/group). *, p < 0.05; **, p < 0.01, Student's t test (A); one-way ANOVA (B–E). The data are shown as the means ± S.E. and are representative of at least three experiments.