Figure 2.

TM7SF2 mRNA is responsive to sterols in human but not hamster cell lines. A, cDNA samples were prepared from CHO-7 or SRD-1 cells treated for 24 h with 5 μm compactin (statin) or 10 μm 25-hydroxycholesterol (25HC) (30). B, Huh7, Be(2)C, and HeLaT cells were treated for 24 h with the following: 5 μm compactin (statin), 10 μm 25-hydroxycholesterol, 10 μm 24(S),25-epoxycholesterol (24,25EC), or 10 μm T0-901317 (60). Total RNA was harvested, and cDNA was prepared. A and B, mRNA levels for TM7SF2 and LBR were measured using qRT-PCR and normalized to PBGD. mRNA levels are relative to the control condition (for A, the CHO-7 control), which was set to 1. Data are presented as mean ± S.E. (error bars) from three independent experiments, each performed in triplicate.