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DHCR14-V5 localizes to the ER, whereas LBR-V5 localizes to the perinuclear region. CHO-EV, CHO-DHCR14-V5, and CHO-LBR-V5 cells were transfected with dsRedER plasmid (an ER marker), prior to staining with anti-V5 and DAPI. Slides were imaged with a Nikon C1 confocal microscope at ×100 for DAPI, dsRedER, and Alexa Fluor® 488.
