Table 1.
E3 ligases identified in DHCR14 and DHCR7 immunoprecipitation
Scores were assigned by MASCOT, and MASCOT scores >30 were used as a threshold for identification. Coverage represents the percentage of the protein that was mapped by found peptides.
| Accession | Gene name | Description | Immunoprecipitation experiment | Found? | Score | Coverage % |
|---|---|---|---|---|---|---|
| Q86TM6 | HRD1 | E3 ubiquitin-protein ligase synoviolin1 (SYVN1) | DHCR7 | Yes | 41.01 | 1.46 |
| DHCR14 | No | |||||
| Q9UKV5 | GP78 | E3 ubiquitin-protein ligase AMFR | DHCR7 | Yes | 266.07 | 7.15 |
| DHCR14 | Yes | 47.84 | 2.80 | |||
| Q9UNE7 | CHIP | E3 ubiquitin-protein ligase CHIP | DHCR7 | Yes | 54.8 | 3.96 |
| DHCR14 | No | |||||
| Q5T447 | HECTD3 | E3 ubiquitin-protein ligase HECTD3 | DHCR7 | Yes | 53.42 | 1.63 |
| DHCR14 | No | |||||
| Q15034 | HERC3 | Probable E3 ubiquitin-protein ligase HERC3 | DHCR7 | Yes | 47.42 | 1.33 |
| DHCR14 | No | |||||
| Q7Z6Z7 | HUWE1 | E3 ubiquitin-protein ligase HUWE1 | DHCR7 | Yes | 137.07 | 0.87 |
| DHCR14 | No | |||||
| 884908087 | Itch | E3 ubiquitin-protein ligase Itchy | DHCR7 | No | ||
| DHCR14 | Yes | 57.93 | 1.73 | |||
| O43164 | PRAJA2 | E3 ubiquitin-protein ligase Praja2 | DHCR7 | Yes | 31.69 | 2.54 |
| DHCR14 | No | |||||
| O00308 | WWP2 | NEDD4-like E3 ubiquitin-protein ligase WWP2 | DHCR7 | Yes | 53.91 | 1.49 |
| DHCR14 | No |