Figure 2.

In vitro activity of HADHB and viperin. a, the thiolase activity of purified recombinant HADHB was assayed in the presence of increasing concentrations of ΔN-viperin. Maximal inhibition was observed at equimolar concentrations of the two enzymes. b, reductive SAM cleavage activity of purified recombinant ΔN-viperin, assayed in the absence or presence of HADHB and/or the cosubstrate CTP (100 μm). The uncoupled rate of SAM cleavage and the rate of SAM cleavage in the presence of CTP are significantly accelerated by HADHB. Values are presented as mean ± S.E. (n = 6). **, p < 0.005; ***, p < 0.001 (Student's t test for independent samples).