Figure 5.

HOTAIR promoted cell invasion through inhibiting WIF‐1 expression and activating Wnt signaling pathway. A, Overexpression of HOTAIR decreased WIF‐1 expression, and silencing of HOTAIR increased WIF‐1 expression in both mRNA and protein levels in LOVO and SW480; *P < .05 and **P < .01 vs Vector. *P < .05 and *P < .05 and **P < .01 vs siNC. (B) Overexpression of HOTAIR promoted the nuclear accumulation of β‐catenin in both LOVO and SW480 cell lines; Scale bar, 200 µm. (C) HOTAIR overexpression (HOTAIR) decreased E‐cadherin expression and increased N‐cadherin expression levels; while the effect was reversed by the application of FH535. *P < .05 and **P < .01 vs Control. *P < .05 and **P < .01 vs HOTAIR