Fig. 1.

Characterize AMPK interactome using TAP-MS analysis. A, AMPK interactome study workflow using a TAP-MS approach. Constructs encoding SFB-tagged AMPK subunits were expressed in HEK293T cells. Cell lysates underwent two steps of affinity purification using streptavidin beads and S-protein agarose beads. The samples were analyzed by LTQ-Orbitrap Velos Pro, and the identified peptides and proteins were further filtered using HCIP analysis, as described in the text. B, Validation of cell lines that stably expressed each of the seven AMPK subunits. C, The identified PSMs for AMPK subunits in each TAP-MS experiment are depicted. The size of the blue cycle reflects the number of identified PSMs.