Fig. 2.

The nuclei of low quality spermatozoa are more susceptible to decondensation following treatment with SDS/EDTA. Following Percoll fractionation A, high- (upper) and low- (lower) quality spermatozoa were challenged with SDS/EDTA then stained with DAPI. Decondensed nuclei are shown (white arrow); scale bar = 5 μm. B, Box and whisker plot of DAPI fluorescent areas from >100 cells in high- and low-quality populations of sperm isolated from three donors and subjected to decondensation (*p < 0.00001).