Fig. 3.

Levels of histone H3 are consistent between high and low quality spermatozoa populations. A, Immunoblot of SDS extracted proteins from high- and low-quality spermatozoa isolated from three ejaculates, probed using anti-H3. Cross-reacting bands were visualized with chemiluminescence after addition of horse-radish peroxidase conjugated secondary antibody. Lower Blot: following recording of chemiluminescence, the nitrocellulose was stripped of primary antibody, re-blocked and probed with anti-α Tubulin. The chemiluminescent signal obtained was used a loading control. B, The abundance of histone H3 according to the SWATH analysis between high- and low-quality sperm cells demonstrates no significant difference between the populations (n = 8, p > 0.05).