Fig. 6.

TOP2A is more abundant in spermatozoa derived from the low quality fractions. Human spermatozoa from individual donors were fractionated through Percoll density gradients. Intact cells were lysed and run into SDS-PAGE. Following transfer, the nitrocellulose was probed with primary antibodies against A, TOP2A (upper blot). Cross-reacting bands were visualized with chemiluminescence after addition of horse-radish peroxidase conjugated secondary antibody. Lower Blot: Following recording of chemiluminescent, the nitrocellulose was stripped of primary antibody, re-blocked and probed with anti-α Tubulin. B, The TOP2A abundance according to the SWATH analysis between high- and low-quality sperm cells demonstrates significantly more abundance within the latter (n = 8, *p = 0.0001).