Table II. The peptide biomarker candidates of M. catarrhalis and the proteins from which they originate.
| Peptide sequence | Number of times detected in number of 33 MS analyses of M. catarrhalis cultures | Number of times detected in unique clinical samples | Protein (GenBank accession number and description) | |
|---|---|---|---|---|
| VVLAGDTVVSDR | 33 | 14 | WP_003666427.1 | TonB-dependent receptor |
| QIVSNAGDEASVIVNEVK* | 33 | 18 | WP_063454121.1 | Chaperonin GroEL |
| AIAQVGSISANSDATIGELISK | 29 | 16 | ||
| ELSNTAAETQPK | 33 | 18 | WP_003659702.1 | 30S ribosomal protein S1 |
| VDATVDAQNPTK | 24 | 16 | WP_003660336.1 | Hypothetical protein |
| QSDVGQLTGK | 5 | 9 | ||
| FNATAALGGYGSK | 31 | 12 | WP_063454085.1 | Cell surface protein |
| THTSALAEENQQASIPR | 33 | 12 | WP_063454087.1 | Cell division protein FtsZ |
| YVVEGANMPLDAQAIDIVR | 17 | 11 | WP_049156084.1 | NADP-specific glutamate dehydrogenase |
| SQIYQTTASVSGAR | 33 | 9 | WP_003657351.1 | Ohr family peroxiredoxin |
| LLNETTGQVVPK | 33 | 8 | WP_003657987.1 | DUF4377 domain-containing protein |
| SSENVVVVSVR | 33 | 10 | WP_063454071.1 | Electron transfer flavoprotein subunit beta |
| AISYGNSADAQPYVGAK | 33 | 10 | WP_003658939.1 | Porin family protein |
| GLPVSNSGAPISVPVGQATLGR | 31 | 8 | WP_003658974.1 | F0F1 ATP synthase subunit beta |
| VNYNGDTDTVTLSGVAK | 33 | 13 | WP_003656943.1 | Peptidoglycan-binding protein LysM |
| AVATQQATVSAEYLQK | 5 | 10 | WP_003657125.1 | ABC transporter substrate-binding protein |
| ADSGLSESEIEEMIR | 32 | 12 | WP_003669031.1 | Molecular chaperone DnaK |
| LGAQEAELVSNSK | 33 | 7 | WP_003660298.1 | CTP synthase |
*This peptide was also found in samples spiked with the fewest number of cells (supplemental Table S2).