Table 2.

PCR primer sequences used in this study

Genes	Accession No.	Sequence (5’→3’)	Size, bp
ZO-1	XM_021098896.1	F:CCAACCATGTCTTGAAGCAGC	215
		R:TGCAGGAGTGTGGTCTTCAC
Occludin	NC_010458.4	F:TCAGGTGCACCCTCCAGATT	169
		R:TATGTCGTTGCTGGGTGCAT
Claudin-1	NC_010455.5	F:AAGGACAAAACCGTGTGGGA	247
		R:CTCTCCCCACATTCGAGATGATT
GAPDH	NM_001206359.1	F:CCAAGGAGTAAGAGCCCCTG	125
		R:AAGTCAGGAGATGCTCGGTG
β-actin	XM_003124280.4	F:CTCCAGAGCGCAAGTACTCC	153
		R:AATGCAACTAACAGTCCGCC

ZO1 Zona occludens protein 1, GAPDH Glyceraldehyde 3-phosphate dehydrogenase