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. Author manuscript; available in PMC: 2020 Mar 2.
Published in final edited form as: Dev Biol. 2019 Oct 11;458(1):64–74. doi: 10.1016/j.ydbio.2019.10.007

Fig. 2. Intermediate region of epiblast is specified towards neural crest cell fate independent of neuroectodermal and mesodermal cell fates.

Fig. 2.

(A) Explants from EGK Stage XII chick embryos, collected after 25h and analyzed for expression of different cell fates; mesodermal (TbxT, (Brachyury) lateral-most), neural (Sox2, medial), and NC (Pax7, intermediate explant) (n = 4). (B) Graphs showing RT-qPCR analysis of twelve explants after 25h of culture, for the expression of Pax7 (NC marker), Sox2/Sox3 (neural marker), and TbxT/Tbx6 (a lateral mesodermal markers). Fold change in expression is represented by normalizing to GAPDH (reference gene) and relative to the positive control region for each gene: cranial neural fold (NF)/neural plate (NP) for Pax7 and Sox2/Sox3 expression and primitive streak (PS) for TbxT/Tbx6. Data from four individual sets of embryos, each with 12 explants and controls, are represented in the RT-qPCR graphs are shown, with error bars representing standard error of mean between four biological replicates (4 embryos).