| Component | Concentration |
|---|---|
| HEPES (Sigma H0887) | 15mM |
| Sodium Bicarbonate (Life Technologies 25080–094) | 3.6mM |
| L-glutamine (Invitrogen 35050–061) | 4mM |
| Insulin (Sigma I6634) | 10 μg/mL |
| Transferrin (Sigma T5391) | 5 μg/mL |
| Cholera Toxin (C8052) | 0.1 μg/mL |
| Epidermal Growth Factor (Corning 354001) | 25ng/mL |
| Bovine Pituitary Extract (Invitrogen 12028–014) | 30 μg/mL |
| Fetal Bovine Serum (ThermoFisher SH3008003HI) | 5% |
| Retinoic Acid (Sigma R2625) | 0.05 μM |
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