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. Author manuscript; available in PMC: 2020 Mar 2.
Published in final edited form as: Cell Rep. 2020 Feb 11;30(6):1848–1861.e5. doi: 10.1016/j.celrep.2020.01.050

Figure 4. ENDU-2 Regulates CTPS-1 Phosphorylation through the PKA Pathway in the Intestine.

Figure 4.

(A) Western blot of the FLAG-tagged CTPS-1 protein. kin-1 RNAi eliminates CTPS-1 phosphorylation.

(B) Expressing dominant-negative PKA in the intestine reduced CTPS-1 phosphorylation. Numbers below the blot indicate the relative signal intensity between the upper and lower CTPS-1 band in each sample.

(C) GFP fluorescence image and quantitative data for the atgl-1::gfp reporter. Expression increased in endu-2(RNAi) animals.

(D) The whole-worm cAMP level was increased in endu-2(−) mutants.

(E) RNAi of acy genes reduced CTPS-1 phosphorylation. Numbers show the relative signal intensity between the upper and lower CTPS-1 bands in each sample.

(F) qPCR results for mRNA levels of the acy genes in WT or endu-2(−) worms. Error bars indicate standard deviation and asterisks indicate significant differences in all panels.

For (C) and (D), Student’s t test, unpaired, two-tailed, α = 0.01. For (F), Student’s t test, unpaired, 2-tailed with Bonferroni correction, α = 0.0018.