(A) Western blot of the FLAG-tagged CTPS-1 protein. hda-1 RNAi drastically reduced CTPS-1 phosphorylation. The CTPS-1P:CTPS-1 ratio is quantified on the right. n = 2. *p < 0.05 (Student’s t test, unpaired, 2-tailed).
(B and C) Fluorescence images (B) and quantitative (C) data for the hda-1::gfp reporter. endu-2(RNAi) increased the expression of the hda-1::gfp reporter in the intestine. *p < 0.01 (Pearson’s chi-square test).
(D) Fluorescence images and quantitative data for the atgl-1::gfp reporter. hda-1 RNAi did not affect the expression of an atgl-1::gfp reporter.
Error bars indicate standard deviation in all of the panels.