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. 2020 Feb 3;10(3):407–413. doi: 10.1002/2211-5463.12794

Figure 3.

Figure 3

PCBP2 depletion affects sortilin mRNA levels. (A, B) Depletion of PCBP1 and PCBP2 in RAW264.7 cells. Cells were transfected with siRNA targeting Pcbp1, Pcbp2, or a control nontargeting siRNA, then harvested 48 h after transfection. Cell lysates were subjected to immunoblotting (A) with the indicated antibodies. Total RNA were subjected to qRT‐PCR (B) to quantify Pcbp1 and Pcbp2 mRNA. Data are the means ± SD (n = 3). *P < 0.01 (two‐way ANOVA). (C) Destabilization of sortilin mRNA by PCBP1 depletion in cells. RAW264.7 cells were transfected with control (siNTS), PCBP1 (siP1), or PCBP2 (siP2) siRNA for 48 h and, then, treated with 1 µg·mL−1 ActD for 9 h. Total RNA were subjected to qRT‐PCR to quantify sortilin mRNA. Data are the means ± SD (n = 3). *P < 0.01 (two‐way ANOVA).