Figure 4.

Zinc ions affect the poly(C)‐binding ability of PCBP2. EMSA analysis of PCBP2. ³²P‐labeled poly(C) oligonucleotide probe (100 nm) was mixed with buffer alone (P) or yeast lysates expressing FLAG‐PCBP2. Samples were separated by PAGE. A 10‐fold molar excess of unlabeled poly(C) oligonucleotide or control mutated oligonucleotide was added as specific competitor (S) and nonspecific competitor (N), respectively. Zinc sulfate and ferrous ammonium sulfate (both 100 μm) with or without 10 mm EDTA were incubated with samples prior to incubation with the probes.